Niagara-on-the-Lake is about 130km from Toronto, reached by the QEW highway to St. Catharines then north on regional roads — around 90 minutes by car. From Niagara Falls, it's 20km north along the Niagara Parkway, a scenic riverside road that passes through the wine country and Queenston Heights.
There is no direct train or bus service from Toronto to the town itself. The Niagara Airbus runs shuttle service from Toronto Pearson Airport. In summer, Niagara-on-the-Lake also receives a seasonal ferry from Youngstown, New York, across the Niagara River.