The gallery occupies the bel piano of the Palazzo Ducale and was assembled in the 19th century from suppressed churches and convents across the Marche. Its prize possessions are three works by Piero della Francesca: the haunting Flagellation of Christ, the Madonna of Senigallia, and the diptych ideal city panel known as the Ideal View of a City.
Beyond Piero, the collection includes Raphael's young portrait of La Muta, Paolo Uccello's Profanation of the Host predella, and works by Titian, Pedro Berruguete, and Justus of Ghent. The Studiolo of Federico is part of the same ticket. The ticket office and entrance are inside the palace courtyard.