Montespertoli is best reached by car. The nearest motorway exits are Firenze Certosa on the A1 (23 km) and Ginestra Fiorentina on the FI-PI-LI dual carriageway (10 km). From the FI-SI dual carriageway, take the San Casciano exit (18 km) or Bargino exit (15 km).
There is no train station in town, but SITA buses run between Florence and Montespertoli several times a day.