Quarto d'Altino is on the Venice–Trieste railway line, served by regional trains from Venice Santa Lucia (about 15 minutes) and Treviso (about 20 minutes). The station is about a kilometre from the museum and town centre, walkable in 15 minutes.
By car, the town is accessible from the A4 motorway (Venice–Trieste direction) at the Quarto d'Altino exit, and is also close to Marco Polo International Airport — about 5 kilometres by road. It makes a practical base if Venice accommodation is full or expensive, with easy train access to the city.