Valle de Guadalupe sits north-east of Ensenada along Mexican Federal Highway 3, the Ruta del Vino. From Ensenada the drive is roughly 30-45 minutes; from Tijuana or the San Diego border, allow about two hours before border delays.
Public transport is limited. If you plan to taste wine, use a tour operator, hire a driver, or arrange taxis carefully in advance. Many winery roads are dirt and can be rough after rain.