Villa Florida sits on Ruta 1, the main paved highway between Asunción (160 km north) and Encarnación (about 200 km south). Long-distance buses on the Asunción–Encarnación service all stop in town; the journey from Asunción takes roughly two and a half hours.
By car, the drive south from Asunción is straightforward on Ruta 1 — paved the whole way, but watch for slow trucks and the occasional checkpoint. There is no airport in Villa Florida; the nearest is Silvio Pettirossi (ASU) in Asunción. Hire cars are widely available in Asunción for the drive down.