The Santuario della Madonna della Querce (Sanctuary of Our Lady of the Oak) sits about a kilometre outside Lucignano's walls, easily reached on foot along a pleasant lane through the farmland. The Renaissance church, probably designed by Vasari or his workshop in the 1550s, is a refined example of the architectural language being developed in Florence at that time.
The interior is simple but elegant, with a single nave and a coffered ceiling. The devotional image of the Virgin — the object of pilgrimage — is housed in an altar niche. The walk out to the sanctuary and back, through olive groves and past views of the Valdichiana, is one of the most pleasant short excursions from Lucignano.