Fort Caroline National Memorial preserves the site of a 16th-century French Huguenot settlement on the south bank of the St. Johns River, about 10 kilometres upstream from the river's mouth. The visitor centre explains the history of the French attempt at colonisation and the subsequent Spanish massacre through exhibits and a well-presented film. Outside, a replica of the original triangular fort stands in a clearing near the river.
The surrounding Timucuan Ecological and Historic Preserve extends across both sides of the river estuary and protects one of the largest remaining undeveloped estuaries on the US Atlantic coast. Trails through the preserve lead to the Ribault Monument, which marks the spot where French explorer Jean Ribault landed in 1562, and to areas of salt marsh, coastal hammock, and tidal flat that give a sense of the landscape the first European settlers encountered. The Spanish Pond Trail is particularly good for birds.